Aircraft are high vibration environments. Every threaded fastener is subject to loosening and must be secured. Acceptable methods include lock washers, lock nuts, cotter pins, safety wire, snap rings, etc. Aviation maintenance 101.
I once forgot to wire the bolt head on an alternator tensioning arm. Took it for a 30 minute shakeout flight. When I landed, the bolt was gone.
This really sucks, but let's be honest: Rob had a hand in his own death. The mod was made to his specs, and he was well known for doing his own maintenance. IMO 99% odds he was the last person to touch that plug.